Orange and grapefruit growers devastated by citrus diseases have new hope: CRISPR gene editing

The company tested for HLB resistance by grafting their CRISPR-edited trees onto HLB-diseased trees and found that the disease did not spread to the edited trees. Credit: Mike Lewis via UC Riverside Center for Invasive Species Research
The company tested for HLB resistance by grafting their CRISPR-edited trees onto HLB-diseased trees and found that the disease did not spread to the edited trees. Credit: Mike Lewis via UC Riverside Center for Invasive Species Research

In the past few decades, two bacterial diseases — Huanglongbing (HLB) and citrus canker— have decimated the Florida citrus industry by killing millions of trees, costing the state billions in lost revenue, and reducing production by 80%. HLB disease has spread to Alabama, California, Georgia, Louisiana, Mississippi, South Carolina, and Texas. There are currently no economical solutions for these industry-threatening diseases. Growers are applying large amounts of pesticide to combat the disease; this is unsustainable and has little to no effect.

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Biotechnology start-up Soil Culture Solutions, LLC (d/b/a Soilcea), in conjunction with the Univ. of Florida (UF), is attempting to solve this problem by developing disease-resistant citrus trees using CRISPR precision breeding. CRISPR precision breeding is a powerful tool for breeding new resistant varieties that the U.S. Dept. of Agriculture (USDA) can classify as non-GMO. This process mimics natural evolution, where edits occur that delete the DNA that causes susceptibility to diseases, allowing the plant to adopt the beneficial change slowly through natural selection.

Yianni Lagos, CEO of Soilcea, says, “We are excited to partner with growers and nurseries to get new canker- and HLB-resistant trees in the field, and to help restore the Florida citrus industry.”

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