Sometimes in science there are unexpected threads tying seemingly very different things together.
Unraveling the knots in these threads can lead to new insights into important developmental processes and mechanisms of disease.
My lab studies epigenomic and transcription factors including a molecule called histone variant H3.3 (more here on H3.3).
H3.3 binds to the actual thread of DNA to create very different kinds of chromatin states than those made by the more traditional canonical histone H3 family members. Think of H3.3 as the unorthodox memberย of the histone H3 family.
Recent studies have indicatedย that H3.3 plays key roles in bothย stem cells and cancer.
Share this article
The two genes that make H3.3 protein, H3f3a and H3f3b, are expressed differentially so cells may make their total pool of H3.3 protein only from the โaโ gene or only the โbโ gene or from both. We knocked out the โbโ gene.
As a result, some genes switched inappropriately into โonโ mode, while others that were supposed to be active were ย switched off. The germ cell DNA was also not packaged properly. The end result was dead or dysfunctional sperm. In addition, earlier on in the spermatogenesis process, specific more primitive germ cell populations in the โbโ knockouts died as well.
Read the full, original story: What do sperm have to do with brain tumors?
