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CRISPR could efficiently improve difficult-to-breed oilseed crops, study shows

Brassica napus, also known as rapeseed, is an important oilseed crop
This article or excerpt is included in the GLP’s daily curated selection of ideologically diverse news, opinion and analysis of biotechnology innovation.

CRISPR/Cas9 is a valuable tool for both basic and applied research that has been widely applied to different plant species. Nonetheless, a systematical assessment of the efficiency of this method is not available for the allotetraploid Brassica napus—an important oilseed crop. In this study, we examined the mutation efficiency of the CRISPR/Cas9 method for 12 genes and also determined the pattern, specificity and heritability of these gene modifications in B. napus.

Collectively, our results demonstrate that CRISPR/Cas9 is an efficient tool for creating targeted genome modifications at multiple loci that are stable and inheritable in Bnapus. These findings open many doors for biotechnological applications in oilseed crops.

Here, we show that CRISPR/Cas9 could specifically and efficiently induce targeted mutations at one locus or multiple loci in the T0 generation of Bnapus and that the mutations were stably inherited into the progeny.

The GLP aggregated and excerpted this article to reflect the diversity of news, opinion and analysis. Read full, original post: CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus
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