The GLP is committed to full transparency. Download and review our 2019 Annual Report

Infographic: Creating artificial human chromosomes

| | November 26, 2019

This article or excerpt is included in the GLP’s daily curated selection of ideologically diverse news, opinion and analysis of biotechnology innovation.

To convert a piece of cloned DNA into a centromere-containing human artificial chromosome (HAC), an array of repeated LacO sequences is incorporated into the DNA. The DNA is then transfected into human cells that have been engineered to express a fusion protein consisting of a LacO binding domain and a factor called HJURP. The fusion protein binds to the LacO domains (1), and incorporates the specialized centromeric histone CENP-A into and along neighboring chromatin (2). In turn, the region of CENP-A-containing chromatin forms a centromere, converting the cloned DNA into a functional self-perpetuating HAC (3).

artchromo hires fin
Image: George Retseck

Read full, original post: Infographic: Building an Artificial Chromosome

Related article:  'Strange' decade gave us CRISPR, gene therapy advances and a Neanderthal genome
News on human & agricultural genetics and biotechnology delivered to your inbox.
Optional. Mail on special occasions.

Send this to a friend