CRISPR 2.0: New gene-editing tools offer plant breeders greater precision

Print Friendly, PDF & Email
vertical farming x

There is no best, only better. This seems truly the case in the history of genome editing technology. When the CRISPR/CAS9 (clustered regularly interspaced short palindromic repeats/CRISPR associated-proteins) system was first developed in 2012-13, dubbed CRISPR 1.0, it was considered the best system ever in genome editing. But no longer.

…. In brief, [CRISPR] is a molecular scissor developed from a bacterial defense system that can be reprogrammed to make a cut across both DNA strains at a target genome site in animal and plant cells.

Although it is still being used widely due to its effectiveness in shutting down genes of interest, CRISPR 1.0 has been exposed with critical limitations, such as an inability to precisely replace, delete, and insert a single or more predetermined DNA base in the genome …. To improve CRISPR 1.0, many different CRISPR/CAS9 variants have been developed and tested, including the latest one, called prime editors.

Related article:  CRISPR-edited crops could go on sale in Japan before end of 2019

Prime editors are a major upgrade of CRISPR 1.0 that can eliminate or minimize the limitations aforementioned, representing an enormous breakthrough in CRISPR/CAS9-based genome editing technology. Many scientists consider prime editors a game changer in genome editing.

…. [P]rime editing could be used to replace a target DNA base with any other single bases, to insert a DNA of one to 44 base pairs (bp), to delete a DNA of one to 80 bp, or to do any combinations of the listed three in human and mouse cells.

Read the original post

Outbreak Featured
Infographic: Autoimmune diseases — 76 identified so far — tend to target women over men. Here is a master list

Infographic: Autoimmune diseases — 76 identified so far — tend to target women over men. Here is a master list

There are many autoimmune diseases, and taken together they affect as much as 4.5 percent of the world’s population. This ...
Are GMOs and pesticides threatening bees?

Are GMOs and pesticides threatening bees?

First introduced in 1995, neonicotinoids ...
glp menu logo outlined

Newsletter Subscription

* indicates required
Email Lists
glp menu logo outlined

Get news on human & agricultural genetics and biotechnology delivered to your inbox.