โThe traditional methods of bee breeding have been around for nearly 100 years,โ said Purdue Universityโsย Brock Harpur, assistant professor ofย entomology. Although breeders have made progress, many of their methods remain unchanged even with the recent availability of affordable gene sequencing.
โThe honeybee genome was sequenced in 2006. There really hasnโt been a lot of bee breeding in this country thatโs been empowered by that resource,โ he said. Harpur aims to change that with Purdueโsย Luiz Brito, associate professor ofย animal sciences, andย Gregor Gorjancย of theย Roslin Instituteย at the University of Edinburgh in Scotland. They seek to validate genomic breeding tools while designing a system that bee breeders can implement themselves.
Their work is among the projects supported by $11.6 million in grants allocated from theย U.S. Department of Agricultureย National Institute of Food and Agricultureย earlier this year to promote healthy pollinator populations. Agricultural pollination production from the beekeeping industry in the U.S. is valued at more than $19 billion annually.
โThat excludes honey production, which is another huge industry,โ Harpur said. โBee breeding is central to that. Beekeepers historically have responded to industry threats with breeding. The goal here is to help them do that better with genomics resources.โ
Five years ago, with genetic samples priced at $400 each, that prospect would have been costly. But the price has dropped to about $50 per sample.ย A 2017 economic case studyย of Canadian honeybee colonies assessed the value of an expensive sequencing technology.
โThey were looking at one trait versus the many weโre hoping to predict,โ Harpur said. โThey showed that if a beekeeper used these more expensive genome-enabled breeding techniques, they could increase their profits by between 9% and 96%. Using genomic technology, we think we can increase the amount of money that beekeepers make.โ
Harpurโs lab, which operates a gene sequencing service for beekeepers, has sequenced nearly 2,000 samples from across the country. โIf you’re a beekeeper, you can send us a bee, and we’ll sequence it and tell you about it and where it’s from,โ he said.
Harpur can use the genotypes from that same database to see how effectively they predict breeding values under ideal conditions. He also uses simulated datasets to explore the statistical validity of his approach on supercomputers at Purdueโsย Rosen Center for Advanced Computing.
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The simulations allow the researchers to assess how many samples are enough to statistically validate their goals.
โIf we have a thousand samples, is that enough?โ Harpur said. โDo we need 10 times more? A hundred times more? And furthermore, if those thousand samples come from 10 different populations, does that matter?โ
The computer simulations also enable the scientists to analyze thousands or millions of honeybee genomes with many different histories and from many different situations to see how well their genomic predictions fit reality. The simulations can even help identify which of the many available genome sequencing methods might work best, Harpur noted.
Harpur works closely with bee breeding organizations nationally, especiallyย the Indiana Queen Breeders Association. Some trials and genomic data collection already have begun. โWe’re going to start local, show that this works and then move to a more global scale,โ he said.
As an evolutionary biologist, Harpur sees the project as a way to answer questions both for bee breeding and for basic science. How does the artificial selection of bee breeding affect bee colonies, which consist of tens of thousands of highly social individuals?
โTheyโre all genetically related, and they all collectively manifest these traits that weโre interested in,โ he said. โWhen I talk about honey production, thatโs not the action of one bee. Thatโs the action of thousands of related bees.
โSo, when you make a prediction on a single genome for a trait that thousands of individuals contributed to, how exactly does that math work out? And how exactly does selection work in a case like that?โ
