CRISPR prime editing: Broad Institute biochemist David Liu discusses pros, cons and off-target effects

p p y unnamed
Image: David Liu

STAT invited readers to submit questions to [biochemist David] Liu on the new [CRISPR prime] technology. He also received some questions directly. From his perch at the Broad Institute of MIT and Harvard, Liu answered:

Zoe A.: Can you please compare the pros and cons of prime editing versus base editing?

[Liu:] We directly compared prime editors and base editors in our study, and found that current base editors can offer higher editing efficiency and fewer indel byproducts than prime editors, while prime editors offer more targeting flexibility and greater editing precision.

Anonymous: Are unintended on- and off-target effects likely to be more or less of an issue with the new method compared with other methods?

Related article:  UK biotech firm poised to test disease-resistant CRISPR bananas and coffee in South America

[Liu:] We show in our study a side-by-side comparison of off-target editing between Cas9 nuclease and prime editing at known Cas9 off-target sites, with the result that prime editing is far less prone to off-target editing at known Cas9 off-target sites. Of course, these observations do not necessarily mean that prime editors will not have their own off-target activities, and additional studies are needed to study this possibility.

Read full, original post: You had questions for David Liu about CRISPR, prime editing, and advice to young scientists. He has answers

Outbreak Daily Digest
Biotech Facts & Fallacies
Talking Biotech
Genetics Unzipped
Video: Test everyone – Slovakia goes its own way to control COVID

Video: Test everyone – Slovakia goes its own way to control COVID

As Europe sees record coronavirus cases and deaths, Slovakia is testing its entire adult population. WSJ's Drew Hinshaw explains how ...
mag insects image superjumbo v

Disaster interrupted: Which farming system better preserves insect populations: Organic or conventional?

A three-year run of fragmentary Armageddon-like studies had primed the journalism pumps and settled the media framing about the future ...
dead bee desolate city

Are we facing an ‘Insect Apocalypse’ caused by ‘intensive, industrial’ farming and agricultural chemicals? The media say yes; Science says ‘no’

The media call it the “Insect Apocalypse”. In the past three years, the phrase has become an accepted truth of ...
globalmethanebudget globalcarbonproject cropped x

Infographic: Cows cause climate change? Agriculture scientist says ‘belching bovines’ get too much blame

A recent interview by Caroline Stocks, a UK journalist who writes about food, agriculture and the environment, of air quality ...
organic hillside sweet corn x

Organic v conventional using GMOs: Which is the more sustainable farming?

Many consumers spend more for organic food to avoid genetically modified products in part because they believe that “industrial agriculture” ...
benjamin franklin x

Are most GMO safety studies funded by industry?

The assertion that biotech companies do the research and the government just signs off on it is false ...

Environmental Working Group: EWG challenges safety of GMOs, food pesticide residues

Known by some as the "Environmental Worrying Group," EWG lobbies for tighter GMO legislation and famously puts out annual "dirty dozen" list of fruits and ...
m hansen

Michael Hansen: Architect of Consumers Union ongoing anti-GMO campaign

Michael K. Hansen (born 1956) is thought by critics to be the prime mover behind the ongoing campaign against agricultural biotechnology at Consumer Reports. He is an ...
News on human & agricultural genetics and biotechnology delivered to your inbox.
Optional. Mail on special occasions.
Send this to a friend